Acrylamide-ES ELISA Test Kit with 0.03ppb High Sensitivity for 1.5 Hours Detection Time and 96T Specification

Intended Use: For the detection of Acrylamide in ground coffee and fried food using the Acrylamide-ES ELISA Kit.

Range of Detection: This method detects 15 ppb to 1,200 ppb in matrix.

5.1 Weigh 1 g of ground coffee sample into a 50 mL conical tube.

5.2 Add 20 mL of boiling water (distilled or deionized) to the sample. Cap the tube tightly.

5.3 Place the sample on an overhead mixer and rotate at 50% speed-setting for 30 minutes.

5.4 Place the sample in a ventilated hood and add 2.5 mL of ammonium hydroxide to the sample (note: only use 2 mL for decaffeinated coffee).

5.5 Place the sample on the overhead mixer and rotate at 50% speed-setting for 30 minutes.

5.6 Centrifuge the sample for 5 minutes at 4,000 rpm.

5.7 Add 1.5 mL of the supernatant to the SLE+ column. Use the column adapter and 10 mL syringe to apply gentle, positive pressure until all of the supernatant passes onto the column bed. DO NOT FORCE THE SUPERNATANT THROUGH THE COLUMN BED—the sample must remain on the column. If the sample flows through the column, the column must be discarded and a new column should be used with a fresh aliquot of supernatant.